Foods of plant origin - Multiresidue methods for the determination of pesticide residues by GC or LC-MS/MS - Part 2: Methods for extraction and clean-up
This European Standard specifies methods for the extraction and clean-up of food samples of plant origin for quantitative determination of pesticide residues. Different solvents can be used for this purpose. These pesticide residues are generally associated with other co-extracted compounds which would interfere in the analysis. To purify the crude extracts to be analysed, several techniques can be used.This European Standard contains the following extraction and clean-up methods that have been subjected to interlaboratory studies and/ or are adopted throughout Europe:- method M: Extraction with acetone and liquid-liquid partition with dichloromethane/ light petroleum, if necessary clean-up on Florisil® ) [1], [2], [3] - method N: Extraction with acetone, liquid-liquid partition with dichloromethane or cyclohexane/ ethyl acetate and clean-up with gel permeation and silica gel chromatography [4], [5] - method P: Extraction with ethyl acetate, and if necessary, clean-up by gel permeation chromatography [6].This European Standard specifies the details of methods M, N and P for the extraction and the clean-up of food samples of plant origin. Several solvents at different volumes are used for extraction. Techniques of clean-up are listed such as liquid-liquid partition, liquid chromatography on various adsorbents and gel permeation chromatography.A table providing the couples (matrix/ pesticide) which have been submitted to collaborative studies and a list of indicative applicability of the method to different pesticides are given for each method, wherever possible.
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