ISO 11816-1|IDF 155-1:2013 specifies a fluorimetric method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution.
The instrument can read activities up to 7 000 milliunits per litre (mU/ l). If the activity is higher than 7 000 mU/ l, it is diluted with alkaline phosphatase-free milk so as to obtain a measurement not higher than 7 000 mU/ l.