Infant formula and adult nutritionals - Determination of fructans - High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) after enzymatic treatment (ISO 22579:2020)
This document specifies a method for the determination of inulin-type fructans (including oligofructose, fructooligosaccharides) in infant formula and adult nutritionals (both powder and liquid) containing 0,03 g/ 100 g to 5,0 g/ 100 g of fructans in the product as prepared ready for consumption.The method has been validated in a multi laboratory study[1] with reconstituted standard reference material (SRM), infant/ adult nutritional formula at a level of 0,204 g/ 100 g, adult nutritionals ready-to-feed (RTF) at levels of 1,28 g/ 100 g and 2,67 g/ 100 g, infant formula RTF at a level of 0,300 g/ 100 g, reconstituted follow-up formula at levels of 0,209 g/ 100 g to 0,275 g/ 100 g, reconstituted infant formula at levels from 0,030 8 g/ 100 g to 0,264 g/ 100 g. During the single laboratory validation study[2], spike-recovery experiments were performed up to 5 g/ 100 g in reconstituted infant formula powders (milk-based, partially hydrolysed milk-based and soy-based), adult nutritional RTF and reconstituted adult nutritional powders.
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